h޴S�j�0����2�X���d��BۇM�J��p�l�d�~5����adA�Ci���@�� Works with current protocols and reagents: Accuri developed the C6 to use the same reagents you currently use on the market-leading cytometers. Select the BD Accuri CFlow file (.c6) that contains the data you want to view with FCS Express. This item has been powered on, but no further testing. %%EOF Accuri C6 is an analyzer equipped with two lasers and 4 color detectors and it has capabilities similar to FACSCalibur. BD Accuri C6 Plus flow cytometer With its compact 11 x 14.75 x 16.5-in footprint, light weight of 30 lb and operational simplicity, the BD Accuri C6 Plus flow cytometer supports a wide array of applications including immunology, cell and cancer biology, plant and microbiology, and industrial applications. Update Driver Software - Cytometer, Successfully Updated Your Driver Software, Figure 5-5.The C6 Software Status Message Box - C6 Is Connected and Ready. If the BD Accuri software displays the message Extra Start-up time needed due to cleaning or improper shut down it will take the instrument several more minutes for the instrument to get to the green-light ready state. 21. Manuals and User Guides for BD Accuri C6. Click here to view the C6 software user guide. Accuri C6 is an "easy to use" flow cytometer which can be connected to an autosampler (48 and 96 well, and 24 tube rack, we do not have one). Such counts are essential in many research and clinical applications, including enumerating leucocytes, B cells, T cells, and platelets in human blood, measuring of … Came with note that it needs repair. Additional laser configurations (2-blue 2-red, 4-blue) are available with the Selectable Lasers Module (Cat. Laboratory Equipment BD CSampler Installation Manual 2 pages. The Accuri C6 cytometer in my lab has some problems, and we need some help. If you are interested in using a fluorochrome that is not listed here with the Accuri C6, please contact the operator.. Click here to view the instrument manual. Compare the new data to that provided with the C6 and email the file created in step 20 to TechSupport@AccuriCytometers.com *Steps may be skipped if you purchased your computer from Accuri with your C6. ۟��@��7����O����vƁ`���OW�p�>����8\:�7k������������>q=���!�\�$/-dzCL��)��"�nÒ��2fVo.C��I�aj���~��`��-`v�T�͖(V�` �㆔����rHf ��d)R�7��V�� ר�Gˌ%b��j�Z��.�f>�Z�i-h�`*G-�mA�S,�1&;e,��R�h�Q�4 Lh&4��{�,a��T���.hݓ�t�D�4,�L��J�'�uI�(Y�22��(�t�O�+L�op���z�￠exy����w�c#s����{�u^�(�����H� The BD Accuri C6 flow cytometer (Figure 2) offers performance, simplicity, and affordability. BD Accuri C6 Software (CFlow Plus) controls the BD Accuri C6 flow cytometer system in order to acquire data, generate statistics, and analyze results. BD Accuri C6 Instrument Manual (31 pages) Flow Cytometer. Instructional video on the basics of using BYU's Accuri C6 cytometer. Only operate the Accuri C6 Flow Cytometer with an original copyrighted version of software authorized by Accuri. The BD Accuri C6 Plus is an ideal benchtop platform for core immunophenotyping studies and is configured for rapid and accurate analysis of up to six parameters at the single-cell level. Accuri C6 flow cytometer fits in any lab and is readily accessible to all researchers—experts and novices alike. Narrated by Dr. Donald Breakwell Filmed and edited by Gabriel Goulding BD Accuri C6 Flow Cytometer Instrument Manual Bd Accuri C6 Pdf User Manuals. x���M��� `(-X�:��&�̑� r�p�N��S�F?�R4�S�^L=�� I��t�_�n@Q4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_`���=��@J�'� �������(�⍴?�aV(�S��'3*{�����f����߫�!cE��+MH4~������U1Qy+��R�L�Vj��\i@���|����f�M�ZY?�`��bJ�ܠD��Ke��@��T`�e���8�HQ�W�8�7�y����R�T���p�]����ޣ�y.� nW�$�����[�}�k�[j�-J�����[�C�+�gA-�G鳅������g��6v���, ����R�s%�Yu���f�y'��8l���N%. The BD Accuri™ C6 is a personal flow cytometer that brings cell analysis within reach by being easy to use, simple to maintain, and affordable. User's Guides and Manuals. Update Driver Software - Cytometer, Second Dialog Box, Figure 5-4. Accuri C6 consumables, manuals, etc. DNA/Cell cycle analysis BD Accuri C6 Plus software files can be exported in FCS 3.1 format for seamless data import into flow cytometry analysis programs such as FCS Express ™ software and FlowJo ™ software. This includes microfuge tubes and tubes made of polystyrene and polypropylene. ... Deionised water is recommended in the C6 manual - try this and it may stop blockages. endstream endobj 2780 0 obj <>stream Install, update, and run anti-virus protection software on a regular basis. This manual provides information on cytometer requirements and specifications, setup, and maintenance. View online or download Bd Accuri C6 Instrument Manual Bd Accuri C6 Manuals | ManualsLib BD Accuri™ C6 Flow Cytometer Optical Filter Guide . The BD Accuri C6 flow cytometer system includes BD Accuri C6 Software for controlling the instrument, generating statistics, and analyzing results. The BD Accuri® C6 is a digital basic flow cytometer, which is equipped with a Blue (488nm) and a Red (640nm) lasers, two light scatter detectors (FSC and SSC), and four fluorescence detectors. The BD Accuri™ C6 Plus personal flow cytometer is the newest generation of the BD Accuri™ platform. ?���[��? So the choice should be addressed by the kind of flow experiments you plan to do. Press the Power button on the BD Accuri C6 instrument face panel. The BD Accrui C6 Cytometer -The cytometer has 2 lasers: -Blue 488nm laser (3 colors) -Red 640 (1 color) -Unlike most cytometers you will encounter, the Accuri does not all you to manually adjust your voltage settings -Instead, the software uses a larger logarithmic … BD Accuri C6 Plus flow cytometer With its compact 11 x 14.75 x 16.5-in footprint, light weight of 30 lb and operational simplicity, the BD Accuri C6 Plus flow cytometer supports a wide array of applications including immunology, cell and cancer biology, plant and microbiology, and industrial applications. BD Accuri™ C6 Compensation Calculator (Microsoft® Excel®) BD Accuri™ C6 Flow Cytometer Optical Filter Guide . It also includes common troubleshooting help and service information. Peristaltic Pumps and Luer Locks, Figure 4-5. !� �XB4��u*��yo7�5�(8.����.�����jo���U߅Ţ?=_��Jk X�3^��=8l�S����6ؽ�6]�w����Wߵ %��S&!bM\w��d�T�����&�B��~��s˟1U�R�l1V&�f�PL�L��K6�c 6�9� Standard fluorochrome in use are identical to FACS Calibur. For Research Use Only. No. x������ PP9R�P������t�Y�a9D��� ` +9|%��; ��0^��O���-�&+�M�n����I��]�: ���bh��*��f��M�=�>�����}'$�� Enhanced sensitivity, reliability, and capabilities bring flow cytometry even more within reach for new and experienced flow cytometry researchers. BD Accuri C6 Plus fluorochrome support All fluorochromes in the table can be detected by the BD Accuri C6 Plus equipped with standard filters. 2774 0 obj <> endobj `� T���4 d*u�y�Dn�cK�u��A�aZC���_��q�� �#��� Installation Dialog Box .....80 Figure C-3. 2804 0 obj <>stream Accuri C6 is a digital PC based bench top analyser, 2 laser system. Such wear includes scratches, scrapes, and general dirtiness. If you will be using a different system, consult the appropriate protocol in our flow cytometry protocols for Multiplex assays page, or contact our Technical Support team at technical@abcam.com. For a more complete set of directions refer to the BD Accuri C6 Flow Cytometer Instrument Manual . %PDF-1.6 %���� 0 Core Size/Flow Rate Combinations.....79 Figure C-1. The DNA histograms produced by the BD Accuri C6 display excellent peak CVs, and this instrument, uniquely, can provide simultaneous linear estimates of nuclear DNA content spanning at least two orders of … Many thanks, Matt . Click here to view the BD Fluorescence Spectraviewer.. The unique fluidics system of the BD Accuri C6 Plus, driven by peristaltic pumps, allows it to determine sample volume and count cells rapidly, directly, and automatically from the software, eliminating laborious plate counts. x���MkA�����dI#��b�!�@K =�BH}JK���z,�;��i��w����+���n����s�ج�b;���H ���r��n�Ypm8I_�À�W�"v����,,{�m��c__$�`�r݀��\NiC�{i\ �p=�ƫ�k.x���8�ႃ3��{a�> �6111111111111111��Ǧ�� y`�B}�@h�K�v�f�)�*Z�6D�&d'��f�z-C�Zn����R73�nǦ3�-m\�v��tN��ƫ�Zij7��um�zc�&��݌�d7�.� ��L�q����5�H=�Y�pQAں΃��^̑�]#�26m�H-���2'd��5g�Z1GZ;��i�� 9kK:::�;+:8��;:��+::X�;*�,v ����ţ����A(� Includes all accessories shown in pictures. You are viewing an Accuri C6 Flow Cytometer. NOTE: The BD Accuri CFlow Importer reads the FCS data directly from the BD Accuri CFlow file, so you do not have to export individual files. endstream endobj 2775 0 obj <>/Metadata 476 0 R/Pages 2772 0 R/StructTreeRoot 613 0 R/Type/Catalog>> endobj 2776 0 obj <>/MediaBox[0 0 612 792]/Parent 2772 0 R/Resources<>/Font<>/ProcSet[/PDF/Text/ImageB/ImageC/ImageI]/XObject<>>>/Rotate 0/StructParents 0/Tabs/S/Type/Page>> endobj 2777 0 obj <>stream BD Accuri C6 Instrument Manual (31 pages), Figure 1-1. the BD Accuri C6 Flow Cytometer System, BD Accuri C6 Flow Cytometer Specifications, Figure 3-1. �e�I�c~w1���0 ��� The BD Accuri™ C6 is a dual laser flow cytometer capable of detecting four distinct fluorochromes simultaneously. Flow cytometry within reach The analytical power and versatility of today’s laser-based flow cytometry systems have unlocked the mysteries of cell biology and empowered entirely new fields of research. BD Accuri C6 flow cytometer for the analysis of plant genome sizes. Along with traditional forward and side scatter, the C6 is an ideal instrument for basic analytical cytometry. A unique, non-pressurized, peristaltic pump system drives the fluidics. endstream endobj 2779 0 obj <>stream The instrument will begin a 5 minute auto start cycle. BD Accuri C6 Flow Cytometer Optics and Fluidics (Top View, Lid Open), Changing the Workstation Computer Settings for Windows ® 7, Figure 3-5. 661085). Since the C6 fluidics system is non-pressurized, any brand of sample tube that is 12 x 75 mm or smaller can be used. �R'�B$K1�#��L�f����2Φ� 5gM�(�,��ycT�}�b��yL]��:y�y�:��~�Kw�s������b\Pע��._H�vtt哂n�mO*���n�s����;;�5����~��;;a��t��~�HX�ÛK Automated Preventive Maintenance Notification Window, Figure 4-4. Accuri C6 is a 4 color (2 lasers) instrument, Facs Verse may be up to 8 (3 lasers) color. 3. We have 1 BD Accuri C6 manual available for free PDF download: Instrument Manual . 2798 0 obj <>/Filter/FlateDecode/ID[<3B235E56AD4D884590D8BFA25210CF82><756487A21B96FC4983BB2B9C4996548C>]/Index[2774 31]/Info 2773 0 R/Length 99/Prev 1549724/Root 2775 0 R/Size 2805/Type/XRef/W[1 3 1]>>stream The software for Accuri C6 (CFlow Plus) is very user-friendly and efficient. Accuri™ C6 Plus BD Biosciences. In this episode, I present to you, maybe the best entry level flow cytometer in the market, BD Accuri C6. The BD Accuri C6 flow cytometer system includes BD Accuri C6 Software for controlling the instrument, generating statistics, and analyzing results. In addition, C6 has a flow metering system for automatic calculation of absolute cell counts. (In this example, the file is FCS Express Import Demo.c6.) 1 The standard configuration of the BD Accuri C6 Plus (as shipped) is “3-blue 1-red” with the 533/30 (formerly 530/30) filter in FL1, 585/40 in FL2, 670 LP in FL3, and 675/25 in FL4. Removing Peristaltic Pump Retainer Clip, Figure 4-6. endstream endobj 2781 0 obj <>stream Overall very good cosmetic condition. Even more colors to choose from with selectable lasers and filter replacement. Figure 1-1. The BD Accuri® C6 personal flow cytometer can simplify cell analysis by counting absolute cell numbers directly (per unit sample volume). Item is sold AS IS. Accuri C6 consumables, manuals, etc. With minor signs of normal wear and tear. Related Manuals for BD Accuri C6 . h�bbd```b`` � ��9������"ɸD�NB��!�6�[��f����3>��������L�\�`��X��?�� �YF endstream endobj 2778 0 obj <>stream Sliding Luer Lock Fittings Off of Pump Head, Figure 5-2. endstream endobj startxref It also includes … ��qq��p�{|�wtxs BD Accuri C6: BMSB J538. y\L }����~*L�l�n}��� 7�x�m����X��gȱ0Z1J50 m��@� �D�1Hğ����H31�m� �� ���' L��� A broad dynamic range of detection makes it easy to analyze cells as varied in size as platelets and eosinophils in the same data file. Operate the instrument as described in this Instruction Manual. Optional filters can be used to increase resolution or to separate fluorochromes with overlapping signals, like GFP and YFP. User Tracking Installer Icon .....80 Figure C-2. 1.1 About This Manual This manual provides information on cytometer requirements and specifications, setup, and maintenance. BD Accuri C6 with plate handler April 2016 This protocol contains instructions for setting up an Accuri C6 with plate handler. BD Accuri C6 Software User Guide - Manual vi 7820095-01 Rev-0 Table B-1. Services available on the BD Accuri: Single or multi-color cellular analysis (phenotyping, intracellular staining, etc.) �! Available to trained users with online booking. h�b```d``ja`c``�� Ā B,@Q�G����^�M�*O�:.:�c�a��"~��!������Q���ȵ�۵8�1}p�l�����C�+x��L��a:_"�����.��r+��枘�! BD Accuri C6 Software provides the following features: a. Tabbed views for collection, analysis, and statistics b. 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